基于几丁质酶基因序列分析鉴定兔源须癣毛癣菌

doi:10.11843/j.issn.0366-6964.2015.08.018

Abstract

Abstract:

It was conducted to identify the gene type of 31 Trichophyton mentagrophytes isolated from rabbits.Samples were collected from infected rabbits for cultivation experiments on Sabouraud′s medium.Isolates were identified by morphology，urease test，hair perforation test and DNA analysis，and then subjected to phenotyping analysis by CHS1 gene sequencing.Results showed that 87.09% (27/31) of the colonies were granular- and powder-like with yellow-brown pigmentation deposition.Numerous grape-like microconidia，spiral hyphae，pectinafe mycelium and club-shaped macroconidia were presented in the culture.Urease test and hair perforation test were positive.The CHS1 sequences of the isolates showed 99.2%-95.4% homology with Trichophyton interdigitale.The results indicate that the isolates obtained in the present study are most likely zoophilic Trichophyton interdigitale.Based on the CHS1 gene variations in sequences，the isolates were divided into 5 genotypes.The type Ⅰ and type Ⅱ were the dominant genotypes，which accounted for 54.84% (17/31) of the isolates.These results suggest that analysis of CHS1 sequences is useful for identification and typing of Trichophyton mentagrophytes.

CLC Number:

S852.66

LIU Yue，LIU Yan-wei，BAI Fu-juan，LIU Na，LIU Bo. Identification of Trichophyton mentagrophytes in Rabbit based on the Chitinase Gene Sequence Analysis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1417-1424.

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References

［1］陈柏教，孙毅，胡小平，等.须癣毛癣菌的形态学及分子生物学鉴定［J］.中国真菌学杂志，2010，5(6):321-326.
CHEN B J，SUN Y，HU X P，et al.Morphology and molecular biological identification of Trichophyton mentagrophytes［J］.Chinese Journal of Mycology，2010，5(6):321-326.(in Chinese)
［2］DRIRA I，HADRICH I，NEJI S，et al.Highly discriminatory variable-number tandem-repeat markers for genotyping of Trichophyton interdigitale strains［J］.J Clin Microbiol, 2014，52(9):3290-3296.
［3］SUN P L，HSIEH H M，JU Y M，et al.Molecular characterization of dermatophytes of the Trichophyton mentagrophytes complex found in Taiwan with emphasis on their correlation with clinical observations［J］.Br J Dermatol，2010，163(6):1312-1318.
［4］DHIB I，FATHALLAH A，CHARFEDDINE I B,et al.Evaluation of Chitine synthase (CHS1) polymerase chain reaction assay in diagnosis of dermatophyte onychomycosis［J］.J Mycol Med，2012，22(3):249-255.
［5］CALDERARO A，MOTTA F，MONTECCHINI S, et al. Identification of dermatophyte species after implementation of the in-house MALDI-TOF MS database［J］.Int J Mol Sci，2014，15(9):16012-16024.
［6］刘彦威，柳焕章，刘建钗，等.河北兔场皮肤病原真菌rDNA-ITS序列分析［J］.中国兽医学报，2013，33(9):1383-1386.
LIU Y W，LIU H Z，LIU J C，et al.Sequences analysis of rDNA ITS of rabbit dermatophytes in Hebei province［J］.Chinese Journal of Veterinary Science, 2013，33(9):1383-1386.(in Chinese)
［7］CAFARCHIA C，IATTA R，LATROFA M S，et al.Molecular epidemiology phylogeny and evolution of dermatophytes［J］. Infect Genet Evol, 2013，20:336-351.
［8］CAFARCHIA C，WEIGL S，FIGUEREDO L A，et al.Molecular identification and phylogenesis of dermatophytes isolated from rabbit farms and rabbit farm workers［J］.Vet Microbiol，2012，154(3-4):395-402.
［9］NENOFF P，KRÜGER C，GINTER-HANSELMAYER G，et al.Mycology-an update.Part 1：Dermatomycoses：causative agents，epidemiology and pathogenesis［J］.J Dtsch Dermatol Ges，2014，12(3):188-209.
［10］GRÄSER Y，SCOTT J，SUMMERBELL R，et al.The new species concept in dermatophytes-a polyphasic approach［J］.Mycopathologia，2008，166(5-6):239-256.
［11］KAWASAKI M，ASO M，INOUE T,et al.Two cases of tinea corporis by infection from a rabbit with Arthroderma benhamiae［J］. Nihon Ishinkin Gakkai Zasshi，2000，41(4)：263-267.
［12］NENOFF P，UHRLAB S，KRÜGER C, et al.Trichophyton species of Arthroderma benhamiae - a new infectious agent in dermatology［J］.J Dtsch Dermatol Ges，2014，12(7)：571-581.
［13］李庆祥，杨国玲，安利佳.须癣毛癣菌表型的研究［J］.中国麻风皮肤病杂志，2007，23(3):209-210.
LI Q X，YANG G L，AN L J.Study on the phenotyping of Trichophyton mentagrophytes［J］.China Journal of Leprosy and Skin Diseases，2007，23(3):209-210.(in Chinese)
［14］FRÉALLE E，RODRIGUE M，GANTOIS N，et al.Phylogenetic analysis of Trichophyton mentagrophytes human and animal isolates based on MnSOD and ITS sequence comparison［J］.Microbiology，2007，153(10):3466-3477.
［15］NINET B，JAN I，BONTEMS O，et al.Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit［J］.J Clin Microbiol，2003，41(2):826-830.
［16］李金海，刘玉美，宫英，等.常见皮肤癣菌的基因分型［J］.青岛大学医学院学报，2007，43(3)：269-270，272.
LI J H，LIU Y M，GONG Y，et al.Establishment of the genotyping method for common derma tophytes［J］.Acta Academiae Medicinae Qingdao Universitatis，2007，43(3)：269-270，272.(in Chinese)

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Identification of Trichophyton mentagrophytes in Rabbit based on the Chitinase Gene Sequence Analysis

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